dextran hydrodynamic radius

This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. 2016;9706:97061E6. Carbohydrate Polymers, 39, 377-380. Advances in Cosmetic Surgery. Appl Biochem Biotechnol. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Google Scholar. The increased TEWL values which were proportional to the number of microspores indicate SC disruption and formation of microchannels in the skin. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). (1958). 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. 2004;25(9):155967. Peptide and protein transdermal drug delivery. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Probiotics Antimicrob Proteins. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. 2009;8(2):1739. Chem. Colloid and Polymer Science, 286, 1223-1231. (2000). After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. The human skins were from the back or thigh of a 24-year-old woman. A single frequency laser is directed to the sample contained in a cuvette. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Alternatives to animal testing: a review. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. sharing sensitive information, make sure youre on a federal FOIA A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Pure Appl. Soc., 16, 3A, 426-433. ; Bataille, B. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Dextran Fractions. eCollection 2015. Journal of Applied Polymer Science, 13, 2031-2032. This study was supported by the LG Electronics. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. The solution was measured using an Anton Paar DMA35N densitometer. Recent Pat Drug Deliv Formul. Dilute solution properties and degree of chain branching for dextran. 20K. * shows a significant difference between the M.W. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. A solvent at this temperature is called a theta solvent [53]. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Drug Deliv. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. Article De Belder, A.N. The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). (1991). Arch Dermatol Res. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Progress in Polymer Science, 30, 38-70. Catiker, E.; Guner, A. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Crini, G. (2005). FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Equivalent Radii and Ratios of Radii from Solution Properties as Indicators of Macromolecular Conformation, Shape, and Flexibility. HSE and DK helped to porate the skin and to measure TEWL. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. Increased epidermal laser fluence through simultaneous ultrasonic microporation. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Would you like email updates of new search results? Macromolecules, 33, 5730-5739. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. 2008;26(11):12618. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. (1954). Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. 4K and M.W. TEWL measurements were taken 30min after microporation of the human and pig skin. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). ; Yevlampieva N.P. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Carbohydrate Research, 340, 811-826. FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). ; Fran, H.P. statement and Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. 2011;16(2):518. J. Phys. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. In fact, short chains do contain minor branching points. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Ind. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Bahary, W.S. eCollection 2018 Feb. Molecules. ; Weisz, J. An official website of the United States government. 8. We observe that this maximum radius is below Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). De Belder, A. N. (2001). pp13-21. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Carbohydrate Polymers, 74, 372-378. Synthesis and characterization of blue dextrans. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Epub 2012 Oct 18. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. National Library of Medicine The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Herwadkar A, Banga AK. Table 2 compares the properties of nanoparticles coated with one or other polymer. (2008). Hydrodynamic radii ( RH) were determined by dynamic light scattering. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Sachdeva V, Banga AK. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. dextran hydrodynamic radius. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Recent developments in polysaccharide-based materials used as adsorbents in wastewater treatment. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Study of temperature influence. MeSH terms Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Keywords: Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Gekko, K. (1971). Walkenhorst, S.; Olivier, R. (2003). The Intrinsic Viscosity of Biological Macromolecules. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Kasaai, Mohammad R. (2012). This conjecture found to be correct. ; Wood, P.J. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. (2013). The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Kasaai, Mohammad R. (2007). Pathan IB, Setty CM. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Molecular weight (M v) and hydrodynamic properties can be calculated from there. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Carbohydrate Polymers 88 1440-1444. Elias, Hans-Georg (1975). Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. Drug deliv. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should (1969). An undergraduate physical chemistry experiment. Carbohydrate Polymers 88 373-381. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents.

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